CHARACTERIZATION OF THE PRODUCTS FORMED IN THE REACTION BETWEEN 1,3-BUTADIENEMONOXIDE AND 2'-DEOXYADENOSINE BY LIQUID-CHROMATOGRAPHY CONTINUOUS-FLOW FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY

被引：4

作者：

KOSTIAINEN, R ^{[1
]}

KOIVISTO, P ^{[1
]}

PELTONEN, K ^{[1
]}

机构：

[1] INST OCCUPAT HLTH,TOPELIUKSENKATU 41 AA,SF-00250 HELSINKI,FINLAND

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 647卷 / 01期

关键词：

D O I：

10.1016/0021-9673(93)83327-O

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

1,3-Butadiene, a widely produced industrial chemical, has recently been identified as a strong rodent carcinogen. Butadiene is metabolized to reactive 3,4-epoxy-1-butene (BM), which may bind to DNA. The reaction between 3,4-epoxy-1-butene and 2'-deoxyadenosine (dAdo) was studied. The reaction was carried out in trifluoroethanol-triethylamine and the reaction mixture was analysed by liquid chromatography-continuous-flow fast atom bombardment mass spectrometry. The recorded total-ion chromatogram showed four peaks. The spectra of the peaks exhibited a protonated molecule of BM adducts of dAdo (m/z 322), indicating formation of different isomers of the adducts. One alkylation site was shown to be at the exocyclic amino group of the 2'-deoxyadenosine.

引用

页码：91 / 94

页数：4

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