IN-VITRO RELAXATION OF PHENYLEPHRINE-CONTRACTED AND ANGIOTENSIN-II-CONTRACTED AORTIC RINGS BY BETA-ESTRADIOL

被引：15

作者：

RAVI, J ^{[1
]}

MANTZOROS, CS ^{[1
]}

PRABHU, AS ^{[1
]}

RAM, JL ^{[1
]}

SOWERS, JR ^{[1
]}

机构：

[1] WAYNE STATE UNIV,DIV ENDOCRINOL & HYPERTENS,DETROIT,MI 48201

来源：

AMERICAN JOURNAL OF HYPERTENSION | 1994年 / 7卷 / 12期

关键词：

VASCULAR SMOOTH MUSCLE; PHENYLEPHRINE; ANGIOTENSIN II; BETA-ESTRADIOL;

D O I：

10.1093/ajh/7.12.1065

中图分类号：

R6 [外科学];

学科分类号：

1002 ; 100210 ;

摘要：

In vivo studies suggest that 17 beta-estradiol (beta E) may regulate vascular tone. Results of recent studies suggest that beta E exerts rapid effects on intracellular calcium, possibly via cell surface receptors, distinct from conventional nuclear receptors for steroids. The present study was designed to determine whether beta E acutely modifies vascular smooth muscle contractile responses to phenylephrine (PE) and angiotensin II (AII). In experiments on tonic responses of aortic rings to 5 x 10(-8) mol/L PE, cumulative additions of beta E reduced tension at concentrations >10(-6) mol/L. Contractile dose responses to PE were determined in rat aortic rings in absence of sex hormones and then after exposure to beta E (5 x 10(-6) mol/L, n = 6) or vehicle (ETOH, n = 6) for 30 min. beta E increased ED(50) and reduced maximal responses. Application of 5 x 10(-6) mol/L beta E for 30 min also reduced the contractile response to 1 mmol/L AII from 69 +/- 4% (vehicle) to 47 +/- 6% (estradiol) of maximal KCl contraction (P <.025, n = 7). These data suggest that beta E acutely attenuates vasoconstrictor responses to PE as well as to AII, possibly by an effect exerted at the cell membrane level.

引用

页码：1065 / 1069

页数：5

共 24 条

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