BARLEY SPIKELET CULTURE - AN EFFECTIVE TOOL FOR THE ANALYSIS OF BIOCHEMICAL EVENTS IN FLOWER DEVELOPMENT

被引：5

作者：

ASTWOOD, JD ^{[1
]}

HILL, RD ^{[1
]}

机构：

[1] UNIV MANITOBA,DEPT PLANT SCI,WINNIPEG,MB R3T 2N2,CANADA

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1995年 / 40卷 / 01期

关键词：

ANTHER; BARLEY; POLLEN; PROTEIN; SPIKELET CULTURE;

D O I：

10.1007/BF00041123

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Immature detached barley spikelets were cultured in wheat spikelet medium. Fertility of cultured barley spikelets was similar to that of cultured wheat spikelets. Barley anther development within cultured spikelets was retarded relative to in planta, but viability of developing pollen, as determined by a fluorochromatic reaction, was similar in vitro and in planta. Protein synthesis by anthers in developing barley spikelets in vitro was maximal during stage 2, when pollen grains were bicellular, and declined as pollen matured. Barley spikelet culture is an effective tool for the analysis of biochemical events in flower development.

引用

页码：93 / 96

页数：4

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