未找到相关数据
DELAYED-TYPE HYPERSENSITIVITY TO A RECOMBINANT MYCOBACTERIAL ANTIGEN, MPB64, IN GUINEA-PIGS SENSITIZED TO MYCOBACTERIUM-TUBERCULOSIS OR MYCOBACTERIUM-BOVIS BCG
被引：28
作者：
HAGA, S
YAMAGUCHI, R
NAGAI, S
MATSUO, K
YAMAZAKI, A
NAKAMURA, RM
机构：
[1] NATL INST HLTH, DEPT BACTERIOL, TOKYO 141, JAPAN
[2] AJINOMOTO CO INC, CENT RES LABS, KAWASAKI, KANAGAWA 210, JAPAN
关键词：
RECOMBINANT MYCOBACTERIAL ANTIGEN;
DELAYED-TYPE HYPERSENSITIVITY;
MPB64;
D O I：
10.1002/jlb.57.2.221
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ;
090102 ;
摘要：
Recombinant MPB64 (rMPB64), a mycobacterial antigen, was obtained from an Escherichia coli clone transformed with a recombinant expression vector, pMAL64c. The rMPB64 was examined for the activity to elicit delayed-type hypersensitivity (DTH) in guinea pigs injected with live Mycobacterium tuberculosis H37Rv or live M. bovis BCG Tokyo. It was found that rMPB64: has the same reactivity as native MPB64 (nMPB64) or MPT64 (nMPT64) and the potency to elicit DTH was 13.4 times higher than that of PPD. Because MPB64 is secreted only by living M. tuberculosis and some strains of BCG, it is possible to use this antigen for the diagnosis of tuberculosis.
引用
收藏
页码：221 / 225
页数：5
相关论文