PHYSIOLOGICAL APPROACH TO HETEROLOGOUS HUMAN SERUM-ALBUMIN PRODUCTION BY KLUYVEROMYCES-LACTIS IN CHEMOSTAT CULTURE

被引：16

作者：

BLONDEAU, K

BOZE, H

JUNG, G

MOULIN, G

GALZY, P

机构：

[1] ECOLE NATL SUPER AGRON MONTIPELLIER,INRA,CHAIR MICROBIOL IND & GENET MICOORGANISMES,F-34060 MONTPELLIER,FRANCE

[2] RHONE POULENC RORER,DEPT BIOTECHNOL,VITRY,FRANCE

来源：

YEAST | 1994年 / 10卷 / 10期

关键词：

YEAST; KLUYVEROMYCES LACTIS; RECOMBINANT; PHOSPHOGLYCERATE KINASE; GLYCOLYSIS; HETEROLOGOUS PROTEIN; RHSA; CHEMOSTAT CULTURE;

D O I：

10.1002/yea.320101006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Production of recombinant human serum albumin (rHSA) controlled by the constitutive promoter phosphoglycerate kinase was studied in Khuyveromyces lactis. It was governed by both cell concentration and glycolytic flow. The triggering of the fermentation metabolism by unfavourable culture conditions (pH, pO(2), D) caused a decrease in the synthesis of the heterologous protein. The highest productivity (75 mg l(-1) per h) and rHSA concentration (62 mg l(-1)) were obtained in chemostat culture with a dilution rate of 0.12 h(-1) and with 38 g l(-1) dry weight.

引用

页码：1297 / 1303

页数：7

共 24 条

[1] OPTIMIZATION OF CANDIDA-TROPICALIS CYTOCHROME-P450ALK GENE-EXPRESSION IN SACCHAROMYCES-CEREVISIAE WITH CONTINUOUS CULTURES [J].

BERETTA, I ;

SANGLARD, D ;

KAPPELI, O ;

FIECHTER, A .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1991, 36 (01) :48-60

[2] MULTIPLE-COPY INTEGRATION OF THE ALPHA-GALACTOSIDASE GENE FROM CYAMOPSIS-TETRAGONOLOBA INTO THE RIBOSOMAL DNA OF KLUYVEROMYCES-LACTIS [J].

BERGKAMP, RJM ;

KOOL, IM ;

GEERSE, RH ;

PLANTA, RJ .

CURRENT GENETICS, 1992, 21 (4-5) :365-370

[3]

BLONDEAU K, 1993, THESIS ENSA MONTPELL

[4] SEQUENCE ORGANIZATION OF THE CIRCULAR PLASMID-PKD1 FROM THE YEAST KLUYVEROMYCES-DROSOPHILARUM [J].

CHEN, XJ ;

SALIOLA, M ;

FALCONE, C ;

BIANCHI, MM ;

FUKUHARA, H .

NUCLEIC ACIDS RESEARCH, 1986, 14 (11) :4471-4481

[5] A HIGH-FREQUENCY TRANSFORMATION SYSTEM FOR THE YEAST KLUYVEROMYCES-LACTIS [J].

DAS, S ;

HOLLENBERG, CP .

CURRENT GENETICS, 1982, 6 (02) :123-128

[6]

DEDEKEN RH, 1966, J GEN MICROBIOL, V5, P106

[7] INFLUENCE OF PH, NITROGEN AND PHOSPHORUS SOURCES ON THE PRODUCTION OF HEPATITIS-B VIRUS PRE-S2 ANTIGEN BY HANSENULA-POLYMORPHA [J].

DEROUBIN, MR ;

CAILAS, MD ;

SHEN, SH ;

GROLEAU, D .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1992, 38 (02) :158-164

[8] STABLE MULTICOPY VECTORS FOR HIGH-LEVEL SECRETION OF RECOMBINANT HUMAN SERUM-ALBUMIN BY KLUYVEROMYCES YEASTS [J].

FLEER, R ;

YEH, P ;

AMELLAL, N ;

MAURY, I ;

FOURNIER, A ;

BACCHETTA, F ;

BADUEL, P ;

JUNG, G ;

LHOTE, H ;

BECQUART, J ;

FUKUHARA, H ;

MAYAUX, JF .

BIO-TECHNOLOGY, 1991, 9 (10) :968-975

[9]

FLER R, 1992, CURR OPIN BIOTECH, V3, P486

[10] TRANSFORMATION OF INTACT YEAST-CELLS TREATED WITH ALKALI CATIONS [J].

ITO, H ;

FUKUDA, Y ;

MURATA, K ;

KIMURA, A .

JOURNAL OF BACTERIOLOGY, 1983, 153 (01) :163-168

← 1 2 3 →