ISOLATION AND CHARACTERIZATION OF AN ALLERGEN FROM SHORT RAGWEED POLLEN

被引：83

作者：

UNDERDOWN, BJ

GOODFRIEND, L

机构：

[1] Medical Research Associate, Medical Research Council of Canada

来源：

BIOCHEMISTRY | 1969年 / 8卷 / 03期

关键词：

D O I：

10.1021/bi00831a031

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An allergenically active protein, antigen Ra.3, was isolated from the aqueous extract of short ragweed pollen by ammonium sulfate precipitation, gel filtration, and ion-exchange chromatography. Antigen Ra.3 was found to be highly purified on the basis of TEAE-cellulose chromatography, polyacrylamide disc electrophoresis, sedimentation velocity ultracentrifugation, gel filtration, and immunodiffusion tests. Antigen Ra.3 was shown to have a molecular weight of 15,000, an [formula omitted] a total hexose + pentose content of 12.4%, and an amino acid composition distinct from the major allergen of ragweed pollen, antigen E. by direct skin, passive sensitization, and cross-neutralization tests, antigens Ra.3 and E differed in allergenic specificity but shared common allergenic determinants. © 1969, American Chemical Society. All rights reserved.

引用

页码：980 / +

页数：1

共 22 条

[1] ULTRAVIOLET ABSORPTION SPECTRA OF PROTEINS AND AMINO ACIDS
BEAVEN, GH
HOLIDAY, ER
[J]. ADVANCES IN PROTEIN CHEMISTRY, 1952, 7 : 319 - 386
[2] BERRENS L, 1962, Allerg Asthma (Leipz), V8, P75
[3] Cohn E. J., 1943, PROTEINS AMINO ACIDS
[4] DISCHE Z, 1949, ARCH BIOCHEM, V22, P169
[5] DISCHE Z, 1929, MIKROCHEMIE, V7, P33
[6] A colorimetric method for the determination of glucosamine and chondrosamine.
Elson, LA
Morgan, WTJ
[J]. BIOCHEMICAL JOURNAL, 1933, 27 : 1824 - 1828
[7] GIBBONS RA, 1966, GLYCOPROTEINS, P29
[8] GOTTSCHALK A, 1966, GLYCOPROTEINS ED, P29
[9] GREENBERG DM, 1951, AMINO ACIDS PROTE ED, P312
[10] HIRS CHW, 1956, J BIOL CHEM, V219, P611

← 1 2 3 →