FURTHER ANALYSIS ON THE STRUCTURAL PROTEINS OF INFECTIOUS PANCREATIC NECROSIS VIRUS

被引：14

作者：

ESTAY, A

FARIAS, G

SOLER, M

KUZNAR, J

机构：

来源：

VIRUS RESEARCH | 1990年 / 15卷 / 01期

关键词：

2-D PAGE; Chemical labelling; Glycosylation; IPNV; Viral protein;

D O I：

10.1016/0168-1702(90)90015-4

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The structural proteins of infectious pancreatic necrosis virus (IPNV) have been analyzed. Two-dimensional gel electrophoresis showed that IPNV proteins are slightly acidic with apparent pIs ranging from 5.8 to 6.6. To identify the IPNV surface-located proteins, purified virus was labelled either with fluorescein isothiocyanate (FITC) or with Na 125I. After analysis by SDS-PAGE, only the major viral protein, VP2, was labelled by either procedure. The accessibility of VP2 to these reagents suggests that this protein is externally located. In addition, using Concanavalin A conjugated with FITC and IPNV labelling with 3H-mannose, evidence is present that VP2 contains carbohydrate residues. © 1990.

引用

页码：85 / 96

页数：12

共 23 条

[1] COMPARISON OF NONPHOSPHORYLATED AND PHOSPHORYLATED SPECIES OF POLYOMAVIRUS MAJOR CAPSID PROTEIN VP1 AND IDENTIFICATION OF THE MAJOR PHOSPHORYLATION REGION [J].