INHIBITION OF GLUCOSE-REGULATED AND HEAT-SHOCK PROTEIN INDUCTION BY LOW-TEMPERATURE

被引:11
作者
LANKS, KW
机构
[1] Department of Pathology, SUNY Health Science Center at Brooklyn, Brooklyn, NY
关键词
Heat shock protein; Low temperature; Protein induction; Stress;
D O I
10.1016/0304-4165(90)90153-N
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The present study evaluating induction of the major stress proteins in the subphysiological temperature range (25-33° C) shows that none of the agents used could effectively induce the heat shock proteins (hsp) or the glucose related protein grp95 at low temperature. However, grp82 was still induced by some amino acide analogs and by glucose deprivation while certain oxygen-regulated proteins were still induced by hypoxia at 25° C. Analogs were incorporated and protein turnover was increased at low temperature even though most stress proteins were not induced. Synthesis of hsps, but not that of grps, was induced if cultures containing analog-substituted proteins were shifted to 37° C. Temperature dependence of hsp induction by arsenite showed a sharp threshold between 30° C and 33° C. Low temperature inhibition of induction points to the existence of a temperature-dependent mechanism operating within the normal physiological temperature range and may be a useful parameter in evaluating proposed mechanisms of stress protein regulation. © 1990.
引用
收藏
页码:62 / 66
页数:5
相关论文
共 19 条
[1]   EXPRESSION OF THE SACCHAROMYCES-CEREVISIAE GLYCOPROTEIN INVERTASE IN MOUSE FIBROBLASTS - GLYCOSYLATION, SECRETION, AND ENZYMATIC-ACTIVITY [J].
BERGH, MLE ;
CEPKO, CL ;
WOLF, D ;
ROBBINS, PW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (11) :3570-3574
[2]   UNCOATING ATPASE IS A MEMBER OF THE 70 KILODALTON FAMILY OF STRESS PROTEINS [J].
CHAPPELL, TG ;
WELCH, WJ ;
SCHLOSSMAN, DM ;
PALTER, KB ;
SCHLESINGER, MJ ;
ROTHMAN, JE .
CELL, 1986, 45 (01) :3-13
[3]   USE OF IMMOBILIZED LACTOPEROXIDASE TO LABEL L-CELL PROTEINS INVOLVED IN ADHESION TO POLYSTYRENE [J].
CHIN, NW ;
LANKS, KW .
JOURNAL OF CELL BIOLOGY, 1980, 85 (02) :402-413
[4]   70K HEAT-SHOCK RELATED PROTEINS STIMULATE PROTEIN TRANSLOCATION INTO MICROSOMES [J].
CHIRICO, WJ ;
WATERS, MG ;
BLOBEL, G .
NATURE, 1988, 332 (6167) :805-810
[5]   THE HEAT-SHOCK RESPONSE [J].
CRAIG, EA .
CRC CRITICAL REVIEWS IN BIOCHEMISTRY, 1985, 18 (03) :239-280
[6]   A SUBFAMILY OF STRESS PROTEINS FACILITATES TRANSLOCATION OF SECRETORY AND MITOCHONDRIAL PRECURSOR POLYPEPTIDES [J].
DESHAIES, RJ ;
KOCH, BD ;
WERNERWASHBURNE, M ;
CRAIG, EA ;
SCHEKMAN, R .
NATURE, 1988, 332 (6167) :800-805
[7]   INDUCTION CHARACTERISTICS OF OXYGEN REGULATED PROTEINS [J].
HEACOCK, CS ;
SUTHERLAND, RM .
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 1986, 12 (08) :1287-1290
[8]   CELLULAR-RESPONSES TO STRESS - COMPARISON OF A FAMILY OF 71-73-KILODALTON PROTEINS RAPIDLY SYNTHESIZED IN RAT-TISSUE SLICES AND CANAVANINE-TREATED CELLS IN CULTURE [J].
HIGHTOWER, LE ;
WHITE, FP .
JOURNAL OF CELLULAR PHYSIOLOGY, 1981, 108 (02) :261-275
[9]  
JOHNSTON D, 1980, J BIOL CHEM, V255, P6975
[10]  
LANKS KW, 1986, CANCER RES, V46, P1382