A CLONED KINETOPLAST DNA MINI-CIRCLE FRAGMENT FROM A LEISHMANIA SPP SPECIFIC FOR POST-KALA-AZAR DERMAL LEISHMANIASIS STRAINS

被引：23

作者：

DASGUPTA, S ^{[1
]}

GHOSH, DK ^{[1
]}

MAJUMDER, HK ^{[1
]}

机构：

[1] INDIAN INST CHEM BIOL, LEISHMANIA GRP, 4 RAJA SC MULLICK RD, JADAVPUR, Kolkata 700032, W BENGAL, INDIA

来源：

PARASITOLOGY | 1991年 / 102卷

关键词：

LEISHMANIA SPP; MINI-CIRCLE; HYBRIDIZATION; DNA PROBES;

D O I：

10.1017/S0031182000062478

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

DNA-DNA hybridization techniques have been found to be very useful in the classification and identification of Leishmania parasites. We report here the cloning of a mini-circle from Leishmania strain UR6 in a plasmid and subcloning of the mini-circle fragments in M13 mp9. Clone MLURk32, containing a 560 bp fragment of mini-circle, has been found to have unique specificity. Application of this specific probe in identifying different Leishmania isolates reveals that the probe reacted only with strains of post-kala-azar dermal leishmaniasis but not with strains or isolates of visceral leishmaniasis.

引用

页码：187 / 191

页数：5

共 25 条

[1] BIOCHEMICAL-IDENTIFICATION OF CUTANEOUS LEISHMANIAS BY ANALYSIS OF KINETOPLAST DNA .1. ULTRASTRUCTURAL AND BUOYANT DENSITY ANALYSIS [J].