A COMPARATIVE-STUDY OF PROTEINS PRESENT IN SWEATING-SICKNESS-INDUCING AND NONINDUCING STRAINS OF HYALOMMA-TRUNCATUM TICKS

被引:9
作者
BURGER, DB
CRAUSE, JC
SPICKETT, AM
NEITZ, AWH
机构
[1] Department of Biochemistry, University of Pretoria, Pretoria
[2] Tick Research Unit, Veterinary Research Institute, Onderstepoort
关键词
D O I
10.1007/BF01268940
中图分类号
Q96 [昆虫学];
学科分类号
摘要
The identification of antigens unique to the positive strain of the tick Hyalomma truncatum is discussed. Cattle were separately immunized against sweating-sickness-positive and sweating-sickness-negative ticks. A study was done to demonstrate the difference in protein patterns of crude salivary-gland extracts between positive and negative ticks. Immunological differences were identified using Western blots. A 32 kDa band was found to be unique to the positive strain.
引用
收藏
页码:59 / 63
页数:5
相关论文
共 7 条
[1]  
Bezuidenhout J.D., Malherbe A., Sweating sickness: a comparative study of virulent and avirulent strains of Hyalomma truncatum, Tick Biology and Control. Tick Research Unit, pp. 7-12, (1981)
[2]  
Kriek N.P.J., The pathology of sweating sickness, (1977)
[3]  
Laemmli U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, pp. 680-685, (1970)
[4]  
Moos M., Nguyen N.Y., Liu T., Reproducible high yield sequencing of proteins electrophoretically separated and transferred to an inert support, J. Biol. Chem., 263, pp. 6005-6008, (1988)
[5]  
Neitz W.O., Studies on the aetiology of sweating sickness, Onderstepoort J. Vet. Res., 27, pp. 197-203, (1956)
[6]  
Neitz W.O., Sweating sickness: The present state of our knowledge, Onderstepoort J. Vet. Res., 23, pp. 3-20, (1959)
[7]  
Van Atel S.R., Reyers F., Oberem P.T., Matthee O., Further studies on the clinical pathology of sweating sickness in cattle, Onderstepoort J. Vet. Res., 54, pp. 45-48, (1987)