CAPTURE IMMUNOASSAY FOR RUMINANT TUMOR-NECROSIS-FACTOR-ALPHA - COMPARISON WITH BIOASSAY

被引：35

作者：

ELLIS, JA

GODSON, D

CAMPOS, M

SILEGHEM, M

BABIUK, LA

机构：

[1] VET INFECT DIS ORG, SASKATOON S7N O10, SK, CANADA

[2] UNIV WYOMING, DEPT VET SCI, LARAMIE, WY 82070 USA

[3] INT LAB RES ANIM DIS, NAIROBI, KENYA

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 1993年 / 35卷 / 3-4期

关键词：

D O I：

10.1016/0165-2427(93)90040-B

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Monoclonal antibodies and IgG purified from rabbit polyclonal antiserum, raised against recombinant bovine tumor necrosis factor-alpha (TNF-alpha), have been employed in ELISA procedures to quantitate bovine TNF-alpha. These antibodies were potent in neutralizing the biological activity of recombinant as well as natural bovine TNF-alpha. The monoclonal antibodies were used as capture antibodies and were either passively adsorbed or covalently linked to ELISA plates. Polyclonal rabbit anti-TNF IgG was used as the detecting antibody in combination with a biotinylated anti-rabbit serum and a streptavidin-horseradish peroxidase conjugate. The detection limit for recombinant TNF-alpha medium was 10 pg ml -1 and in bovine or ovine serum was 35 pg ml-1. A good correlation was found between the ELISA and the WEHI- 1 64 Clone 13 biologic assay when TNF-alpha was measured in medium containing serum or in serum. This capture ELISA was also capable of detecting ovine, but not porcine. TNF in supernatants from cultures of lipopolysaccharide-stimulated pulmonary alveolar macrophages.

引用

页码：289 / 300

页数：12

共 19 条

[1] CHARACTERIZATION OF RECEPTORS FOR HUMAN-TUMOR NECROSIS FACTOR AND THEIR REGULATION BY GAMMA-INTERFERON [J].