LIQUID-CHROMATOGRAPHIC ATMOSPHERIC-PRESSURE CHEMICAL IONIZATION MASS-SPECTROMETRIC ANALYSIS OF SYNTHETIC ELASTASE INHIBITOR PEPTIDE

被引：4

作者：

KASUYA, F

IGARASHI, K

FUKUI, M

FUKUMORI, Y

TOKUMITO, H

FUKUDA, T

TSUDA, Y

OKADA, Y

机构：

[1] Faculty of Pharmaceutical Science, Kobe-gakuin University, Arise, Kobe, 651-21, Ikawadani, Nishi-ku

来源：

BIOLOGICAL MASS SPECTROMETRY | 1992年 / 21卷 / 10期

关键词：

D O I：

10.1002/bms.1200211006

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 [生物物理学];

摘要：

A liquid chromatographic/atmospheric pressure chemical ionization mass spectrometric method for the determination of tert-butyloxycarbonyl-Tyr-Leu-Val-CH2Cl (Boc-YLV-CH2Cl) was investigated. Boc-YLV-CH2Cl was eluted from a Cosmosil 5C-8 column in a solvent system of 0.1% acetic acid-methanol (30:70, v/v). The positive ion spectrum of Boc-YLV-CH2Cl showed a quasi-molecular ion at m/z 526 with fragment ions which provided structural information. Boc-YLV-CH2Cl was purified from blood and tissue samples with Sep-Pak C18 cartridges. The analysis in biological samples was performed by focusing the characteristic ion at m/z 526. The calibration graphs were linear over the concentration range studied (injected amounts of 9.5-190 pmol). The method was applied to the determination of Boc-YLV-CH2Cl in biological samples.

引用

页码：500 / 504

页数：5

共 10 条

[1]

MASS-SPECTROMETRY WITH ION SOURCES OPERATING AT ATMOSPHERIC-PRESSURE [J].