A NOVEL CATIONIC AMPHIPHILE FOR TRANSFECTION OF MAMMALIAN-CELLS

被引：82

作者：

RUYSSCHAERT, JM

ELOUAHABI, A

WILLEAUME, V

HUEZ, G

FUKS, R

VANDENBRANDEN, M

DISTEFANO, P

机构：

[1] FREE UNIV BRUSSELS,CHIM BIOL LAB,B-1640 RHODE ST GENESE,BELGIUM

[2] FREE UNIV BRUSSELS,INST PHARM,CHIM GEN LAB,B-1050 BRUSSELS,BELGIUM

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 203卷 / 03期

关键词：

D O I：

10.1006/bbrc.1994.2372

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe here a new cationic amphiphile, N-t-butyl-N'-tetradecyl-3-tetradecylaminopropionamidine (diC(14)-amidine), which interacts with plasmid DNA and generates hydrophobic stable complexes resistant against DNase I, In partition experiments between two non-miscible phases, DNA was transferred into an organic phase upon complex formation with diC(14)-amidine-containing vesicles. Finally, vesicles made of a diC(14)-amidine and phosphatidylethanolamine (PE) (1:1, mol:mol) mixture or pure diC(14)-amidine were efficient in mediating (CHO) and suspension (K562) cell lines, using the chloramphenicol acetyltransferase (CAT) gene as reporter. 1994 Academic Press, Inc.

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页码：1622 / 1628

页数：7

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