Summary. The column chromatography of the partially purified lectin showed two peaks and anti‐A agglutinin was present in the first peak having 22.4 S, 15.4 S and 6.9 S. Second peak having 4.2 S was nonspecific. Further studies on the ‘specific’ preparation from column chromatography showed that lectin was more specific for hog A than neopeptone A substance and was not affected by mercaptoethanol treatment. Immunoelectrophoresis showed variations in the A substance from hog and neopeptone. © 1968 Blackwell Publishing Ltd