ANALYSIS OF POLIOVIRUS-SPECIFIC RNA IN INFECTED HELA CELLS BY POLYACRYLAMIDE GEL ELECTROPHORESIS

被引：29

作者：

NOBLE, J

KASS, SJ

LEVINTOW, L

机构：

[1] Department of Microbiology, University of California Medical Center, San Francisco

来源：

VIROLOGY | 1969年 / 37卷 / 04期

关键词：

D O I：

10.1016/0042-6822(69)90271-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The three forms of poliovirus-specific RNA were resolved by electrophoresis in 2.25% polyacrylamide gels. Treatment of infected cells by either phenol or sodium dodecyl sulfate and pronase yielded the same pattern of virus-specific RNA. Mature poliovirus particles also migrated in a characteristic zone in the gel, separate from the three species of RNA. A method for determining the relative base composition of RNA eluted from gels is described. © 1969.

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页码：535 / &

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