CHOLESTEROL-BIOSYNTHESIS IN A VARIETY OF CULTURED-CELLS - LACK OF CORRELATION BETWEEN SYNTHESIS AND ACTIVITY OF 3-HYDROXY-3-METHYLGLUTARYL COENZYME A REDUCTASE CAUSED BY DEXAMETHASONE

被引：32

作者：

JOHNSTON, D ^{[1
]}

CAVENEE, WK ^{[1
]}

RAMACHANDRAN, CK ^{[1
]}

MELNYKOVYCH, G ^{[1
]}

机构：

[1] UNIV KANSAS,MED CTR,DEPT MICROBIOL,KANSAS CITY,KS 66103

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 572卷 / 01期

基金：

美国国家卫生研究院;

关键词：

(Cultured cell); Cholesterol biosynthesis; Dexamethasone; Hydroxymethylglutaryl-CoA reductase;

D O I：

10.1016/0005-2760(79)90215-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In a variety of cell lines grown in the presence of 10-6 M dexamethasone, the incorporation of acetate into cellular cholesterol was partially suppressed. However, the activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase (mevalonateiNADP+ oxidoreductase (CoA-acylating), EC 1.1.1.34) was either significantly elevated or remained unchanged providing evidence that under certain conditions 3-hydroxy-3-methylglutaryl coenzyme A reductase activity is not a valid measure of cholesterogenesis. © 1979.

引用

页码：188 / 192

页数：5

共 11 条

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[2] CAVENEE WK, 1977, J BIOL CHEM, V252, P3272
[3] REGULATION OF CHOLESTEROL-BIOSYNTHESIS IN HELA S3G CELLS BY SERUM-LIPOPROTEINS - DEXAMETHASONE-MEDIATED INTERFERENCE WITH SUPPRESSION OF 3-HYDROXY-3-METHYLGLUTARYL COENZYME-A REDUCTASE
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JOHNSTON, D
MELNYKOVYCH, G
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (05) : 2103 - 2107
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[5] KANDUTSCH AA, 1977, J BIOL CHEM, V252, P409
[6] STEADY-STATE OF 3-HYDROXY-3-METHYLGLUTARYL COENZYME REDUCTASE-ACTIVITY IN CULTURED RAT-LIVER CELLS AND INCREASE OF ITS ACTIVITY BY DEXAMETHASONE
LIN, RC
SNODGRASS, PJ
[J]. FEBS LETTERS, 1977, 83 (01) : 89 - 92
[7] INHIBITION OF CHOLESTEROL-BIOSYNTHESIS IN HELA-CELLS BY GLUCOCORTICOIDS
MELNYKOVYCH, G
MATTHEWS, E
GRAY, S
LOPEZ, I
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1976, 71 (02) : 506 - 512
[8] NORDSTROM JL, 1977, J BIOL CHEM, V252, P8924
[9] COORDINATE REPRESSION OF CHOLESTEROL-BIOSYNTHESIS AND CYTOPLASMIC 3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE BY GLUCOCORTICOIDS IN HELA-CELLS
RAMACHANDRAN, CK
GRAY, SL
MELNYKOVYCH, G
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1978, 189 (01) : 205 - 211
[10] RODWELL VW, 1973, ADV ENZYMOL RAMB, V38, P373

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