EVIDENCE FOR AN ALKALINE PROTEASE IN VACCINIA VIRUS

被引：11

作者：

ARZOGLOU, P ^{[1
]}

DRILLIEN, R ^{[1
]}

KIRN, A ^{[1
]}

机构：

[1] UNIV LOUIS PASTEUR,INSERM,U74,PATHOGENIE INFECT VIRALES RECH GRP,F-67000 STRASBOURG,FRANCE

来源：

VIROLOGY | 1979年 / 95卷 / 01期

关键词：

D O I：

10.1016/0042-6822(79)90416-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Purified vaccinia virions (Lister strain) were found to contain a proteolytic activity that can use exogenous proteins as a substrate once the virions are disrupted at pH 10.6. Assays of proteolytic activity were carried out with labeled cellular proteins bound to an insoluble support. Purified viral cores obtained after Nonidet-P40 and 2-mercaptoethanol treatment of virus retained the activity. Temperature and pH dependence were observed as well as an inhibitory effect of heavy metal ions. © 1979.

引用

页码：211 / 214

页数：4

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