OXYGEN-UPTAKE BY ENTRAPPED HYBRIDOMA CELLS

被引：23

作者：

WOHLPART, D ^{[1
]}

GAINER, J ^{[1
]}

KIRWAN, D ^{[1
]}

机构：

[1] UNIV VIRGINIA, CTR BIOPROC DEV, DEPT CHEM ENGN, CHARLOTTESVILLE, VA 22903 USA

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1991年 / 37卷 / 11期

关键词：

HYBRIDOMAS; IMMOBILIZATION; OXYGEN; RESPIRATION;

D O I：

10.1002/bit.260371110

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Oxygen consumption by hybridoma cells immobilized in 1- and 3.9-mm-diameter calcium alginate beads was measured. The entrapped cells consumed oxygen at about 10-mu-mol/min per 10(9) cells, regardless of the bead size and cell loading. In contrast, the same cells in suspension culture respire at specific rates of 3-8-mu-mol/min per 10(9) cells (depending on the cell density). The growth rate of the immobilized cells was significantly reduced, while specific antibody production was comparable to that of free cells.

引用

页码：1050 / 1053

页数：4

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