A REFOCUSED AND OPTIMIZED HNCA - INCREASED SENSITIVITY AND RESOLUTION IN LARGE MACROMOLECULES

被引：69

作者：

FARMER, BT

VENTERS, RA

SPICER, LD

WITTEKIND, MG

MULLER, L

机构：

[1] BRISTOL MYERS SQUIBB,PHARMACEUT RES INST,PRINCETON,NJ 08543

[2] DUKE UNIV,MED CTR,DEPT BIOCHEM,DURHAM,NC 27710

来源：

JOURNAL OF BIOMOLECULAR NMR | 1992年 / 2卷 / 02期

关键词：

3D NMR SPECTROSCOPY; REFOCUSED HNCA QUADRUPLE RESONANCE; MODIFIED INEPT SUBSEQUENCE; HNCA CORRELATION; SYNCHRONOUS H-1 BROAD-BAND DECOUPLING;

D O I：

10.1007/BF01875530

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 3D optimized, refocused HNCA experiment is described. It is demonstrated to yield a dramatic increase in sensitivity when applied to [C-13, N-15]-labeled human carbonic anhydrase II, a 29-kDa protein. The reasons for the gain in sensitivity are discussed, and 3 distinct areas for further development are indicated.

引用

页码：195 / 202

页数：8

共 13 条

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