ISOLATION OF RIBOSOMAL-SUBUNITS CONTAINING INTACT RIBOSOMAL-RNA FROM HUMAN PLACENTA - ESTIMATION OF FUNCTIONAL-ACTIVITY OF 80S RIBOSOMES

被引：112

作者：

MATASOVA, NB ^{[1
]}

MYLTSEVA, SV ^{[1
]}

ZENKOVA, MA ^{[1
]}

GRAIFER, DM ^{[1
]}

VLADIMIROV, SN ^{[1
]}

KARPOVA, GG ^{[1
]}

机构：

[1] ACAD SCI USSR, INST BIOORGAN CHEM, NOVOSIBIRSK 630090, USSR

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 198卷 / 02期

关键词：

Amino acids - RNA - Biosynthesis;

D O I：

10.1016/0003-2697(91)90416-Q

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have elaborated a method for the isolation of ribosomal subunits from fresh unfrozen human placenta containing intact rRNA and a complete set of ribosomal proteins. Activity of 80S ribosomes obtained by reassociation of 40S and 60S subunits in nonenzymatic poly(U)-dependent binding of Phe-tRNAPhe was equal to 80% (above 1.5 mol[14C]Phe-tRNAPhe is coupled to 1 mol of ribosomes). The activity of 80S ribosomes in poly(U)-directed synthesis of polyphenylalanine was tested in a polysome-free protein-synthesizing system from rabbit reticulocytes. About 100 mol of phenylalanine residue was polymerized by a mole of ribosomes at a rate of 0.83 residues per minute in this system (2 h, 37°C). © 1991.

引用

页码：219 / 223

页数：5

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