ENZYMATIC-REACTIONS IN AQUEOUS-ORGANIC MEDIA .17. OPTICAL RESOLUTION OF AMINO-ACID ESTERS BY ENZYMATIC-HYDROLYSIS IN ORGANIC-SOLVENTS

被引：15

作者：

TOMIUCHI, Y ^{[1
]}

OHSHIMA, K ^{[1
]}

KISE, H ^{[1
]}

机构：

[1] UNIV TSUKUBA, INST MAT SCI, TSUKUBA, IBARAKI 305, JAPAN

来源：

BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN | 1992年 / 65卷 / 10期

关键词：

D O I：

10.1246/bcsj.65.2599

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Enantiospecific hydrolysis of DL-tyrosine ethyl ester was carried out using alpha-chymotrypsin (CT) as a catalyst in organic solvents, and L-tyrosine was obtained with high optical purity. Activity and enantiospecificity of CT were found to be greatly altered by water content in the reaction media, and generally high enantiospecificity was observed at low water contents. Many of natural and unnatural amino acid esters were resolved by hydrolysis in organic solvents using CT, subtilisin Carlsberg, or subtilisin BPN' as catalysts.

引用

页码：2599 / 2603

页数：5

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