SEQUENCE OF THE BOVINE CD18-ENCODING CDNA - COMPARISON WITH THE HUMAN AND MURINE GLYCOPROTEINS

被引：51

作者：

SHUSTER, DE ^{[1
]}

BOSWORTH, BT ^{[1
]}

KEHRLI, ME ^{[1
]}

机构：

[1] USDA ARS, NATL ANIM DIS CTR, METAB DIS & IMMUNOL RES UNIT, POB 70, AMES, IA 50010 USA

来源：

GENE | 1992年 / 114卷 / 02期

关键词：

INTEGRINS; LEUKOCYTE ADHESION DEFICIENCY; ADHESION; DEFICIENCY;

D O I：

10.1016/0378-1119(92)90586-E

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The bovine cDNA (CD18) encoding CD18, a cell-surface glycoprotein involved in multiple leukocyte functions, was sequenced and compared with the human and murine sequences. Portions of the 5'- and 3'-untranslated regions of the nucleotide sequences are conserved among the three species, including a 3' A+T-rich region believed to regulate mRNA stability and translational efficiency. The 2833-bp bovine sequence coded for a protein of 769 amino acids (aa). Overall, the deduced aa sequences were > 80% identical among the three species. The aa 96-389 and those in the cytoplasmic domain were very highly conserved with approx. 95% aa identity. All Cys residues and potential Asn-glycosylation sites present in the bovine sequence were also present in the human and murine sequences. The aa identity was also found in those regions where mutations were found to cause the genetic disease, leukocyte adhesion deficiency. These data identify functionally important regions of the CD18 mRNA and protein.

引用

页码：267 / 271

页数：5

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