GLYCOSYLATION OF THE GLP-1 RECEPTOR IS A PREREQUISITE FOR REGULAR RECEPTOR FUNCTION

被引：27

作者：

GOKE, R

JUST, R

LANKATBUTTGEREIT, B

GOKE, B

机构：

[1] Clinical Research Unit for Gastrointestinal Endocrinology, Department of Internal Medicine, Philipps-University of Marburg

来源：

PEPTIDES | 1994年 / 15卷 / 04期

关键词：

GLP-1; GLYCOPROTEIN; RECEPTOR; TUNICAMYCIN; RINM5F CELLS;

D O I：

10.1016/0196-9781(94)90095-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The GLF-1 receptor on RINm5F cells is a glycoprotein with a M(r) of 63,000. Treatment of the receptor with glycopeptidaee F generated a protein with a M(r), of 51,000, indicating that the GLP-1 receptor contains N-linked glycans. Tunicamycin pretreatment concentration-dependently decreased GLP-1 binding to RINm5F cells due to a decreased receptor number without change of receptor affinity. Tunicamycin exerted no effect on the GLP-1 receptor mRNA expression. The stimulation of cAMP production was decreased in tunicamycin-treated cells. Our data show that glycosylation of the GLP-1 receptor is a precondition for regular receptor function.

引用

页码：675 / 681

页数：7

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