SEPARATION AND CHARACTERIZATION OF ARABIDOPSIS-THALIANA PROTEINS BY 2-DIMENSIONAL GEL-ELECTROPHORESIS

被引：76

作者：

KAMO, M

KAWAKAMI, T

MIYATAKE, N

TSUGITA, A

机构：

[1] SCI UNIV TOKYO,BIOSCI RES INST,NODA,CHIBA 278,JAPAN

[2] SCI UNIV TOKYO,FAC PHARMACEUT SCI,NODA,CHIBA 278,JAPAN

[3] SCI UNIV TOKYO,DEPT APPL BIOL SCI,NODA,CHIBA 278,JAPAN

来源：

ELECTROPHORESIS | 1995年 / 16卷 / 03期

关键词：

2-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS; ARABIDOPSIS THALIANA; GEL IMAGE ANALYSIS; N-TERMINAL SEQUENCES; DEBLOCKING BY HYDRAZINE VAPOR;

D O I：

10.1002/elps.1150160169

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Arabidopsis (Arabidopsis thaliana) proteins were isolated from five tissues (leaf, stem, root, seed and callus), and separated by two-dimensional gel electrophoresis (2-DE). 2-DE was carried out by immobilized pH gradient (IPG) in the first dimension, and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in the second dimension. With the aid of comigrated five-marker proteins, the patterns of 2-DE gels for each tissue were graphically combined by a computer into a single synthetic image for the integrated Arabidopsis protein spots. The protein spot images, altogether 4763, were characterized by both molecular mass and isoelectric point. Partial amino(N)-terminal sequences of 101 protein spots were analyzed by Edman degradation. Fifty seven proteins were partially sequenced and 46 proteins appeared to have blocked N-termini. Deblocking by hydrazine vapor was carried out on 14 proteins and two of them were found to be pyroglutamyl-blocked N-termini. Forty seven new proteins were found by the present investigation.

引用

页码：423 / 430

页数：8

共 23 条

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