EPIDERMAL GROWTH-FACTOR STIMULATES MITOGEN-ACTIVATED PROTEIN-KINASE BY A PKC-DEPENDENT PATHWAY IN HUMAN KERATINOCYTES

被引:20
作者
MITEV, V
LE PANSE, R
COULOMB, B
MITEVA, L
HOUDEBINE, LM
机构
[1] HOP HENRI MONDOR, DERMATOL LAB, INSERM, U312, F-94010 CRETEIL, FRANCE
[2] HOP ST LOUIS, F-75010 PARIS, FRANCE
[3] INRA, BIOL CELLULAIRE & MOLEC LAB, F-78352 JOUY EN JOSAS, FRANCE
关键词
D O I
10.1006/bbrc.1995.1330
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
`Epidermal growth factor (EGF),20 ng/ml,stimulated myelin basic protein (MBP) phosphorylation in crude extracts from human keratinocyte primary cultures. In order to identify the involved kinases, we separated by fast protein liquid chromatography proteins participating in MBP phosphorylation. We detected three MBP kinase activities in the keratinocyte crude extracts. The first MBP kinase activity was the only one stimulated by EGF and reacted with anti-mitogen-activated protein kinase (MAPK) antiserum recognising p42(mapk) and p44(mapk) isoforms. However, when protein kinase C (PKC) was either inhibited by the PKC inhibitor GF 109203X or depleted by a prolonged TPA treatment, the stimulation of MBP phosphorylation by EGF was strongly inhibited. The second MBP kinase activity eluted was due to a PKC isoform reacting with an anti-PKC zeta antibody, and the third was not identified. With this work, we have thus shown that, in human keratinocytes, EGF activates MAPK activity by a PKC-dependent pathway. (C) 1995 Academic Press, Inc.
引用
收藏
页码:245 / 252
页数:8
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