SPECTROPHOTOMETRIC ASSAY FOR AMIKACIN USING PURIFIED KANAMYCIN ACETYLTRANSFERASE

被引：14

作者：

SCARBROUGH, E ^{[1
]}

WILLIAMS, JW ^{[1
]}

NORTHROP, DB ^{[1
]}

机构：

[1] UNIV WISCONSIN,SCH PHARM,MADISON,WI 53706

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1979年 / 16卷 / 02期

关键词：

D O I：

10.1128/AAC.16.2.221

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A rapid spectrophotometric assay has been developed for measuring the concentrations of amikacin and related antibiotics in serum. The assay uses a purified enzyme form R-factor E. coli which acetylates amikacin with the production of coenzyme A, the latter in turn being reacted with a sulfhydryl reagent to produce stoichiometric amounts of a sensitive chromophore, that is measured in the visible spectrum. The system complements an earlier assay for gentamicin-related antibiotics thereby facilitating the rapid measurement of the concentrations of all clinically important aminoglycosides in serum.

引用

页码：221 / 224

页数：4

共 12 条

[1]

BENVENISTE R, 1971, BIOCHEMISTRY-US, V10, P1787

[2] SELECTIVE ENZYME PURIFICATION BY AFFINITY CHROMATOGRAPHY [J].