MULTIVARIATE-ANALYSIS OF CARBOHYDRATE DATA FROM LIPOPOLYSACCHARIDES OF ACTINOBACILLUS-(HAEMOPHILUS)-ACTINOMYCETEMCOMITANS, HAEMOPHILUS-APHROPHILUS, AND HAEMOPHILUS-PARAPHROPHILUS

被引:10
作者
BRONDZ, I
OLSEN, I
机构
[1] NATL INST OCCUPAT HLTH,DEPT RES,UMEA,SWEDEN
[2] UNIV OSLO,FAC DENT,DEPT MICROBIOL,OSLO 3,NORWAY
来源
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY | 1990年 / 40卷 / 04期
关键词
D O I
10.1099/00207713-40-4-405
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The taxonomic distinction between Actinobacillus (Haemophilus) actinomycetemcomitans and Haemophilus aphrophilus and the taxonomic distinction between H. aphrophilus and Haemophilus paraphrophilus have been questioned. This study was done to determine whether multivariate statistical analyses of carbohydrate data from lipopolysaccharides could be used to distinguish between these closely related species. Lipopolysaccharides were extracted with phenol-water and purified. Carbohydrates were assessed by using gas chromatography and gas chromatography-mass spectrometry after methanolysis and derivatization with trifluoroacetic acid anhydride. The lipopolysaccharides from all of the species contained rhamnose, fucose, galactose, glucose, L-glycero-D-mannoheptose, and glucosamine plus galactosamine, but in varying amounts. A. actinomycetemcomitans and H. paraphrophilus also contained D-glycero-D-mannoheptose, while H. aphrophilus did not. Sample- and variable-oriented principal-component analyses of the carbohydrate data clearly distinguished among A. actinomycetemcomitans, H. aphrophilus, and H. paraphrophilus. Soft independent modelling of class analogy showed that no sample in the A. actinomycetemcomitans class fell within the 95% confidence limits of the H. aphrophilus class. H. paraphrophilus fell outside both classes.
引用
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页码:405 / 408
页数:4
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