PLASMA OCHRATOXIN A LEVELS IN 3 SWEDISH POPULATIONS SURVEYED USING AN ION-PAIR HPLC TECHNIQUE

被引：135

作者：

BREITHOLTZ, A ^{[1
]}

OLSEN, M ^{[1
]}

DAHLBACK, A ^{[1
]}

HULT, K ^{[1
]}

机构：

[1] ROYAL INST TECHNOL,DEPT BIOCHEM & BIOTECHNOL,S-10044 STOCKHOLM 70,SWEDEN

来源：

FOOD ADDITIVES AND CONTAMINANTS | 1991年 / 8卷 / 02期

关键词：

OCHRATOXIN-A; HUMAN PLASMA; DAILY INTAKE; MYCOTOXIN; ANALYSIS;

D O I：

10.1080/02652039109373968

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A new HPLC method for the analysis of ochratoxin A in plasma samples is described. The analysis is performed at an alkaline pH using an ion-pair technique, fluorescence detection at an excitation wavelength 380 nm, and an emission wavelength 420 nm. The detection and quantification limits are 0.02 ng and 0.05 ng ochratoxin A/ml plasma, respectively. The method was used to determine the ochratoxin A content of human plasma samples, collected in three districts of Sweden. The Visby district had a significantly higher proportion of ochratoxin A positive samples and higher levels than the other two districts-Uppsala and Ostersund. The calculated daily intake of ochratoxin A in the Visby district (0.35 ng/kg body weight), exceeds the lower tolerable daily intake (TDI) value suggested by Kuiper-Goodman and Scott (1989). The calculated daily intake by the population on the mainland of Sweden (0.04 ng/kg body weight) is below the proposed TDIs.

引用

页码：183 / 192

页数：10

共 30 条

[1]

Bauer J., Gareis M., Ochratoxin A in der Nahrungsmittelkette, Journal of Veterinary Medicine Series B, 34, pp. 613-627, (1987)

[2]

Bendele A.M., Carlton W.W., Krogh P., Lillehoj E.B., Ochratoxin A carcinogenesis in the (C57BL/6J X C3H)F1 mouse, Journal of the National Cancer Institute, 75, pp. 733-742, (1985)

[3]

Candlish A.A.G., Stimson W.H., Smith J.E., Determination of ochratoxin A by monoclonal antibody-based enzyme immunoassay, Journal of the Association of Official Analytical Chemists, 71, pp. 961-964, (1988)

[4]

Castegnaro M., Bartsch H., Chernozemsky I., Endemic nephropathy and urinary-tract tumors in the Balkans, Cancer Research, 47, pp. 3608-3609, (1987)

[5]

Chelkowski J., Samson R.A., Wiewiorowska M., Golinski P., Ochratoxin A formation by isolated strains of the conidial stage of Aspergillus glaucus Link ex Gray (= Eurotium herbariorum Wiggers Link ex Gray) from cereal grains, Die Nahrung, 31, pp. 267-269, (1987)

[6]

Frisvad J.C., The connection between the Penicillia and Aspergilli and mycotoxins with special emphasis on misidentified isolates, Archives of Environmental Contamination and Toxicology, 18, pp. 452-467, (1989)

[7]

Frohlich A.A., Marquardt R.R., Bernatsky A., Quantitation of ochratoxin A: Use of reverse phase thin-layer chromatography for sample cleanup followed by liquid chromatography or direct fluorescence measurement, Journal of the Association of Official Analytical Chemists, 71, pp. 949-953, (1988)

[8]

Fuchs R., Hult K., Peraica M., Radic B., Plestina R., Conversion of ochratoxin C into ochratoxin A in vivo, Applied and Environmental Microbiology, 48, pp. 41-42, (1984)

[9]

Hagelberg S., Fuchs R., Hult K., Toxicokinetics of ochratoxin A in several species and its plasmabinding properties, Journal of Applied Toxicology, 9, pp. 91-96, (1989)

[10]

Holmberg T., Breitholtz A., Bengtsson A., Hult K., Ochratoxin A in swine blood in relation to moisture content in feeding barley at harvest, Acta Agriculturae Scandinavica, 40, pp. 201-204, (1990)

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