FACTOR VIII-C ASSAY - INFLUENCE OF BUFFER COMPONENTS USED IN IMMUNOAFFINITY CHROMATOGRAPHY FOR PURIFICATION OF FACTOR-VIII VON-WILLEBRAND-FACTOR

被引：3

作者：

KOOPS, K ^{[1
]}

HOFF, HS ^{[1
]}

HEETHUIS, A ^{[1
]}

DAS, PC ^{[1
]}

SIBINGA, CTS ^{[1
]}

机构：

[1] RED CROSS BLOOD BANK,GRONINGEN,NETHERLANDS

来源：

THROMBOSIS RESEARCH | 1994年 / 74卷 / 04期

关键词：

IMMUNE AFFINITY PURIFIED FVIII/VWF; FVIII-C ASSAY; ONE-STAGE; CHROMOGENIC SUBSTRATE; BUFFER COMPONENTS;

D O I：

10.1016/0049-3848(94)90150-3

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A monoclonal antibody purified factor VIII concentrate containing FVIII/vWF complex has been assayed by one-stage clotting (CA) and chromogenic substrate (CSA) methods. The influences of potassium iodide (KI) and albumin in combination with predilution buffers, standards and storage of samples have been examined. These components are compared for their effect on FVIII potencies in final product and in-process controls. FVIII:C purified by immunoaffinity chromatography can not be measured reliably by CA or CSA, because of KI which interfere on the assay. Overall yield of FVIII, efficiency of IAC step and purity of FVIII can be determined by assaying the desalted samples.

引用

页码：347 / 354

页数：8

共 14 条

[1]

BARROWCLIFFE TW, 1983, THROMB HAEMOSTASIS, V50, P697

[2] REPRODUCIBILITY OF ONE-STAGE, 2-STAGE AND CHROMOGENIC ASSAYS OF FACTOR-VIII ACTIVITY - A MULTICENTER STUDY [J].