GENOME SIZE VARIATION IN PISUM-SATIVUM

被引:170
作者
GREILHUBER, J
EBERT, I
机构
关键词
PISUM SATIVUM L; GENOME SIZE VARIATION; FEULGEN CYTOPHOTOMETRY;
D O I
10.1139/g94-092
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Pisum sativum L. is one of the plant species where infraspecific genome size variation, up to 1.29-fold between cultivars, has been reported. The present investigation deals with a Feulgen cytophotometric analysis of this phenomenon in 25 wild accessions, landraces, and cultivars of widely different geographic origin. Differences between accessions were maximally 1.054-fold in single experiments but proved to be nonreproducible upon repeated measurements. Seedlings of the same accession often differed significantly, up to 1.056-fold, but values from root and shoot tips in one individual were not significantly correlated, indicating the absence of true genome size variation between plants. Upon calibration against Allium cepa a 1C value of 4.42 pg is estimated for Pisum sativum. Altogether the data suggest that, contrary to the divergence in the literature data and recent reports on DNA content variation, the pea has a stable genome size.
引用
收藏
页码:646 / 655
页数:10
相关论文
共 44 条
[1]  
BACHMANN K, 1985, EVOLUTION GENOME SIZ, P267
[3]   CYTOPHOTOMETRIC FACTORS CAUSING APPARENT DIFFERENCES BETWEEN FEULGEN DNA CONTENTS OF DIFFERENT LEUKOCYTE TYPES [J].
BEDI, KS ;
GOLDSTEIN, DJ .
NATURE, 1974, 251 (5474) :439-440
[4]   NUCLEAR-DNA AMOUNTS IN ANGIOSPERMS [J].
BENNETT, MD ;
SMITH, JB .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1991, 334 (1271) :309-345
[5]   VARIATION IN GENOMIC FORM IN PLANTS AND ITS ECOLOGICAL IMPLICATIONS [J].
BENNETT, MD .
NEW PHYTOLOGIST, 1987, 106 (01) :177-200
[6]   NUCLEAR-DNA AMOUNTS IN ANGIOSPERMS [J].
BENNETT, MD ;
SMITH, JB .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1976, 274 (933) :227-274
[7]   NUCLEAR DNA CONTENT AND MINIMUM GENERATION TIME IN HERBACEOUS PLANTS [J].
BENNETT, MD .
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES, 1972, 181 (1063) :109-+
[8]  
BENNETT MD, 1985, PLANT GENETICS, P283
[9]   DNA CONTENT POLYMORPHISM AND TISSUE-CULTURE REGENERATION IN CARIBBEAN PINE [J].
BERLYN, GP ;
ANORUO, AO ;
BECK, RC ;
CHENG, JP .
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1987, 65 (05) :954-961
[10]   INTRASPECIFIC VARIATION OF NUCLEAR-DNA CONTENT IN PLANT-SPECIES [J].
CAVALLINI, A ;
NATALI, L .
CARYOLOGIA, 1991, 44 (01) :93-107