PROTEINASE INHIBITION OF THE DETECTION OF LISTERIA-MONOCYTOGENES IN MILK USING THE POLYMERASE CHAIN-REACTION

被引：115

作者：

POWELL, HA ^{[1
]}

GOODING, CM ^{[1
]}

GARRETT, SD ^{[1
]}

LUND, BM ^{[1
]}

MCKEE, RA ^{[1
]}

机构：

[1] INST FOOD RES, NORWICH LAB, NORWICH RES PK, NORWICH NR4 7UA, NORFOLK, ENGLAND

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1994年 / 18卷 / 01期

关键词：

D O I：

10.1111/j.1472-765X.1994.tb00802.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The direct detection, using the polymerase chain reaction (PCR), of Listeria monocytogenes added to cows' milk was inhibited at some milk concentrations. This inhibitor was moderately heat-stable. Inhibition could be prevented by the addition of Bovine Serum Albumin (BSA) or proteinase inhibitors to the PCR and the evidence suggests that the inhibitor was plasmin.

引用

页码：59 / 61

页数：3

共 9 条

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