ONE-HOUR DOWNWARD CAPILLARY BLOTTING OF RNA AT NEUTRAL PH

This report describes a setup for the downward capillary blotting of RNA with the use of 10X SSC as a transfer solution. The setup is composed of a stack of blotting papers, hybridization membrane, and agarose gel. Two layers of blotting paper connect the stack with two reservoirs containing transfer solution. Using this setup, blotting of RNA fragments (<7.5 kb) can be completed in 1 h. If necessary, the blotting time can be expanded from 1 to 18 h without decrease in hybridization efficiency of RNA. (C) 1994 Academic Press, Inc.