CLOSE COLOCALIZATION OF CD4 AND A SERINE ESTERASE TRYPTASE TL2 ON THE CELL-SURFACE OF HUMAN MONOCYTOID AND CD4+ LYMPHOID-CELLS

被引：4

作者：

INOUE, M

HOSHINO, T

FUKUMA, T

NIWA, Y

KIDO, H

机构：

[1] UNIV TOKUSHIMA,INST ENZYME RES,DIV ENZYME CHEM,TOKUSHIMA 770,JAPAN

[2] KURUME UNIV,SCH MED,DEPT PARASITOL,FUKUOKA 830,JAPAN

[3] KURUME UNIV,SCH MED,DEPT IMMUNOL,FUKUOKA 830,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 201卷 / 03期

关键词：

D O I：

10.1006/bbrc.1994.1857

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tryptase TL2, a serine esterase in the membrane of human monocytoid and CD4+ lymphoid cells, specifically binds to the V3 domain of HIV-1 gp120. Here we report that monoclonal antibodies against CD4 that recognize the epitope interacting with gp120 specifically blocked the immunostaining of cell-surface tryptase TL2, although the antibody does not crossreact with tryptase TL2. Down-regulation of cell-surface CD4 induced by HIV-1 Nef prevented this blocking effect. These data suggest that CD4 is closely co-localized with tryptase TL2 on the cell surface and that regulation of the expression of tryptase TL2 is not associated with that of CD4. (C) 1994 Academic Press, Inc.

引用

页码：1390 / 1395

页数：6

共 12 条

[1] SPECIFIC CELL-SURFACE REQUIREMENTS FOR THE INFECTION OF CD4-POSITIVE CELLS BY HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 AND TYPE-2 AND BY SIMIAN IMMUNODEFICIENCY VIRUS [J].