AUTOMATED ION-EXCHANGE CHROMATOGRAPHY OF URONIC ACIDS AND URONIC ACID CONTAINING OLIGOSACCHARIDES

被引：52

作者：

FRANSSON, LA

RODEN, L

SPACH, ML

机构：

[1] Department of Pediatrics, the La Rabida-University of Chicago Institute, University of Chicago, Chicago

[2] Department of Biochemistry, the La Rebida-University of Chicago Institute, University of Chicago, Chicago

[3] the Joseph P. Kennedy, Jr. Mental Retardation Research Center, University of Chicago, Chicago

来源：

ANALYTICAL BIOCHEMISTRY | 1968年 / 23卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0003-2697(68)90362-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An automated anion-exchange chromatographic procedure for the separation of uronic acids and uronic acid containing oligosaccharides is described. The method has been applied, in particular, to the separation and quantitation of l-iduronic and d-glucuronic acid in hydrolyzates of dermatan sulfate, and the hydrolysis conditions required for optimum yield of uronic acids have been determined. Whereas uronic acids and aldobiuronic acids were successfully separated by elution with formic acid, hyaluronic acid oligosaccharides and, especially, the highly charged chondroitin sulfate oligosaccharides were more conveniently resolved in a chloride system by elution with a nonlinear LiCl gradient. This system was also employed for the separation of unsaturated and saturated disaccharides obtained by chondroitinase digestion of chondroitin and chondroitin sulfate. The effluent from the columns was analyzed by the automated orcinolsulfuric acid method of the Technicon sugar chromatography system. © 1968.

引用

页码：317 / &

共 25 条

[1]

BROWN AH, 1946, ARCH BIOCHEM, V11, P269

[2]

CIFONELLI J. A., 1966, CARBOHYD RES, V2, P150

[3]

DZIEWIATKOWSKI D, 1962, BIOCHIM BIOPHYS ACTA, V56, P167, DOI 10.1016/0006-3002(62)90542-5

[4] SEPARATION OF ACIDIC OLIGOSACCHARIDES BY GEL FILTRATION [J].