ISOLATION AND CHARACTERIZATION OF BABY HAMSTER KIDNEY (BHK-21) CELL MODULATOR PROTEIN

被引:11
作者
YERNA, MJ
HARTSHORNE, DJ
GOLDMAN, RD
机构
[1] Department of Biological Sciences, Carnegie-Mellon University, Pittsburgh
关键词
D O I
10.1021/bi00571a019
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A Ca2+-dependent modulator protein has been isolated from BHK-21 cells. The purification requires heat treatment, ion-exchange chromatography, and gel filtration. The protein appears homogeneous on sodium dodecyl sulfate-polyacrylamide and isoelectric focusing gels. The protein comigrates with purified smooth muscle and brain modulators. BHK-21 modulator is characterized by a high content of aspartic and glutamic acids and by a high phenylalanine/ tyrosine ratio. It lacks both cysteine and tryptophan. The protein is effective in activating brain-modulator-deficient phosphodiesterase. It can also be used in assay systems to generate Ca2+-sensitive actin activation of both BHK-21 and smooth muscle myosins. Therefore, it is proposed that the BHK-21 modulator protein is a component of the Ca2+-dependent mechanism involved in the regulation of actin-myosin interactions in BHK-21 cells. © 1979, American Chemical Society. All rights reserved.
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页码:673 / 678
页数:6
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