A QUANTITATIVE STUDY OF BIOSYNTHESIS OF CYTOCHROME C

被引：30

作者：

KADENBAC.B

机构：

[1] Institut für Physiologsche Chemie, Physikalische Biochemie, Universität München, München, 8

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1969年 / 10卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1969.tb00691.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sucrose‐density gradient experiments have shown it impossible to isolate microsomes from rat liver homogenate free from contaminated mitochondrial cytochrome c. The incorporation of l‐[14C]lysine into cytochrome c from rat liver mitochondria and microsomes in vivo has been studied. The kinetics of the specific radioactivities of free l‐lysine, that of microsomal cytochrome c and that of mitochondrial cytochrome c show precursor‐product relationships. From the kinetics of radioactivity of free l‐lysine and microsomal cytochrome c, the true pool‐size of the latter was calculated to be 1.02 nmoles/g microsomal protein, that is about 1/270 of total rat liver cytochrome c. The turnover‐time of cytochrome c in the endoplasmic reticulum was found to be 102 min. From this data a turnover‐time of total rat liver cytochrome c of 19 days and a half‐life of 13.2 days was calculated. Copyright © 1969, Wiley Blackwell. All rights reserved

引用

页码：312 / &

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