THE ASSOCIATION OF GLYCOSOMAL ENZYMES AND MICROTUBULES - A PHYSIOLOGICAL PHENOMENON OR AN EXPERIMENTAL ARTIFACT

被引：18

作者：

BALABAN, N ^{[1
]}

GOLDMAN, R ^{[1
]}

机构：

[1] WEIZMANN INST SCI, DEPT MEMBRANE RES, IL-76100 REHOVOT, ISRAEL

来源：

EXPERIMENTAL CELL RESEARCH | 1990年 / 191卷 / 02期

关键词：

D O I：

10.1016/0014-4827(90)90008-X

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Subpellicular microtubules isolated from Trypanosoma brucei parasites were fractionated on a phosphocellulose column, and the trypanosomal p52 microtubule-associated protein was eluted along with two other proteins of 41 and 36 kDa. These proteins were found to be the glycosomal enzymes aldolase (41 kDa) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 36 kDa) by enzyme activity, antibody cross-reaction, and N-terminal sequencing. These enzymes were coprecipitated with tubulin in the presence of taxol, and aldolase had the capacity to polymerize tubulin and crosslink microtubules. Immunolocalization of anti-aldolase and anti-GAPDH antibodies did not show an interaction between these enzymes and the subpellicular microtubules. The question whether the copurification of aldolase and the subpellicular microtubules could reflect a physiological phenomenon or may be an experimental artifact is discussed. © 1990.

引用

页码：219 / 226

页数：8

共 32 条

[1] BINDING OF GLYCOLYTIC ENZYMES TO STRUCTURE PROTEINS OF MUSCLE [J].