OLFACTORY SCHWANN-CELLS ARE DERIVED FROM PRECURSOR CELLS IN THE OLFACTORY EPITHELIUM

被引:129
作者
CHUAH, MI
AU, C
机构
[1] Department of Anatomy, Chinese University of Hong Kong, Sha Tin New Town
关键词
OLFACTORY SCHWANN CELLS; EXPLANT CULTURE; GLIAL FIBRILLARY ACIDIC PROTEIN;
D O I
10.1002/jnr.490290206
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
In this morphological and immunohistochemical study we show that olfactory schwann cells (OSC) are derived from precursor cells residing in the olfactory epithelium. During development, they migrate out of the epithelium and extend processes to ensheath the olfactory axons. Olfactory mucosa from E14 rat embryos and juvenile rats were treated with trypsin-pancreatin to remove the underlying connective tissue. The epithelial explant was then maintained for two days in culture, during which cells migrated out from the explant. Among them were spindly bipolar cells which were identified as OSC by their positive immunoreaction for glial fibrillary acidic protein, ultrastructure, and association with growing axons. Axonal growth was significantly more profuse in the embryonic explants, in which the polarity of the OSC was oriented parallel with the axons. Ultrastructural observations showed that ensheathment of the bundles of axons resembled those in vivo.
引用
收藏
页码:172 / 180
页数:9
相关论文
共 28 条
[1]   SCHWANN-CELLS OF THE OLFACTORY NERVES CONTAIN GLIAL FIBRILLARY ACIDIC PROTEIN AND RESEMBLE ASTROCYTES [J].
BARBER, PC ;
LINDSAY, RM .
NEUROSCIENCE, 1982, 7 (12) :3077-3090
[2]   NEURON SCHWANN-CELL INTERACTION IN BASAL LAMINA FORMATION [J].
BUNGE, MB ;
WILLIAMS, AK ;
WOOD, PM .
DEVELOPMENTAL BIOLOGY, 1982, 92 (02) :449-460
[3]  
BUNGE MB, 1983, DEV REGENERATING VER, P71
[4]   ANALYSIS OF NEUROGENESIS IN A MAMMALIAN NEUROEPITHELIUM - PROLIFERATION AND DIFFERENTIATION OF AN OLFACTORY NEURON PRECURSOR INVITRO [J].
CALOF, AL ;
CHIKARAISHI, DM .
NEURON, 1989, 3 (01) :115-127
[5]   BIOSYNTHESIS OF TYPE-IV COLLAGEN BY CULTURED RAT SCHWANN-CELLS [J].
CAREY, DJ ;
ELDRIDGE, CF ;
CORNBROOKS, CJ ;
TIMPL, R ;
BUNGE, RP .
JOURNAL OF CELL BIOLOGY, 1983, 97 (02) :473-479
[6]   DIFFERENTIAL OUTGROWTH OF RETINAL NEURITES ON PURIFIED EXTRACELLULAR-MATRIX MOLECULES [J].
CARRI, NG ;
PERRIS, R ;
JOHANSSON, S ;
EBENDAL, T .
JOURNAL OF NEUROSCIENCE RESEARCH, 1988, 19 (04) :428-439
[7]   RADIAL GLIA OF DEVELOPING HUMAN-FETAL SPINAL-CORD - GOLGI, IMMUNOHISTOCHEMICAL AND ELECTRON-MICROSCOPIC STUDY [J].
CHOI, BH .
DEVELOPMENTAL BRAIN RESEARCH, 1981, 1 (02) :249-267
[8]   OLFACTORY MARKER PROTEIN IS PRESENT IN OLFACTORY RECEPTOR-CELLS OF HUMAN FETUSES [J].
CHUAH, MI ;
ZHENG, DR .
NEUROSCIENCE, 1987, 23 (01) :363-370
[9]  
CUSCHIERI A, 1975, J ANAT, V119, P277
[10]   ELECTRON MICROSCOPIC OBSERVATIONS OF THE OLFACTORY MUCOSA AND OLFACTORY NERVE [J].
DELORENZO, AJ .
JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY, 1957, 3 (06) :839-&