VITAMIN-B12 PRODUCTION BY ACETOBACTERIUM SP AND ITS TETRACHLOROMETHANE-RESISTANT MUTANTS

被引：18

作者：

INOUE, K

KAGEYAMA, S

MIKI, K

MORINAGA, T

KAMAGATA, Y

NAKAMURA, K

MIKAMI, E

机构：

[1] AGCY IND SCI & TECHNOL,FERMENTAT RES INST,TSUKUBA,IBARAKI 305,JAPAN

[2] DAICEL CHEM IND LTD,TSUKUBA RES CTR,TSUKUBA,IBARAKI 305,JAPAN

[3] DAICEL CHEM IND LTD,ABOSHI KU,HIMEJI,HYOGO 67112,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1992年 / 73卷 / 01期

关键词：

D O I：

10.1016/0922-338X(92)90238-P

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A strictly anaerobic, homoacetogenic bacterium strain 69, which produces high amounts of intracellular vitamin B12, was isolated from sea sediment. Taxonomical and physiological studies revealed that the isolate should be classified in the genus Acetobacterium. The isolate grown on methanol produced ca. H mg of cyanocobalamin per gram dry cells after 7-d cultivation. Tetrachloromethane (TCM) resistant mutants, strain 69-7 and 69-23, were obtained from the isolate by ethylmethanesulfonate treatment, and produced ca. 23 mg of cyanocobalamin per gram dry cells for strain 69-7 and 20 mg for strain 69-23 in the presence of 10-mu-M of TCM.

引用

页码：76 / 78

页数：3

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