DISTRIBUTION OF 5'-NUCLEOTIDASE IN MUSCLE OF SOME MARINE FISHES

被引:9
作者
MARSENO, DW
HORI, K
MIYAZAWA, K
机构
[1] Department of Food Science, Faculty of Applied Biological Science, Hiroshima University, Higashi-Hiroshima 724, Japan
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1992年 / 102卷 / 02期
关键词
D O I
10.1016/0305-0491(92)90118-B
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A preliminary examination for the purification and characterization of 5'-nucleotidase of fish muscle was carried out and the following results were obtained: 1. The activities of 5'-nucleotidase in the muscles of marine vertebrates and invertebrates (total 11 species) were determined. The highest activity of 5'-nucleotidase was found in Blackrock fish Sebastes inermis, which was then used as a material for estimation of subcellular distribution and solubilization of the enzyme. 2. The 5'-nucleotidase of ordinary muscle of the fish Sebastes inermis was found in nuclear, microsomal and cytosolic fractions. About half of the total activity was found in the nuclear fraction, whereas the highest specific activity was observed in the microsomal fraction. 3. Complete solubilization of the enzyme was attained by using a high concentration of detergent such as Triton X-100, CHAPS, octylglucoside, octylthioglucoside and sodium deoxycholate, suggesting that the enzyme was tightly bound to the membrane. 4. Based on the results of solubility and stability tests, Triton X-100 seemed suitable for solubilizing 5'-nucleotidase from the membrane. 5. Microsomal 5'-nucleotidase was an Mg2+-activated enzyme, and no inactivation was observed up to 50 mM of Mg2+.
引用
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页码:247 / 253
页数:7
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