ZNF75 - ISOLATION OF A CDNA CLONE OF THE KRAB ZINC-FINGER GENE SUBFAMILY MAPPED IN YACS 1 MB TELOMERIC OF HPRT

被引：19

作者：

VILLA, A ^{[1
]}

ZUCCHI, I ^{[1
]}

PILIA, G ^{[1
]}

STRINA, D ^{[1
]}

SUSANI, L ^{[1
]}

MORALI, F ^{[1
]}

PATROSSO, C ^{[1
]}

FRATTINI, A ^{[1
]}

LUCCHINI, F ^{[1
]}

REPETTO, M ^{[1
]}

SACCO, MG ^{[1
]}

ZOPPE, M ^{[1
]}

VEZZONI, P ^{[1
]}

机构：

[1] WASHINGTON UNIV,CTR GENET MED,DEPT MOLEC MICROBIOL,ST LOUIS,MO 63110

来源：

GENOMICS | 1993年 / 18卷 / 02期

关键词：

D O I：

10.1006/geno.1993.1459

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have previously mapped a zinc finger genomic motif (ZNF75) to the Xq26 cytogenetic band by using a hybrid panel. Here, we report the isolation of the transcribed counterpart in a cDNA clone and its further localization. The cDNA clone, from a lung fibroblast library, is assembled from three exons, including a 289 amino acid (AA) long open reading frame containing a recently described motif, the Kruppel-associated box, 42 AA long, in exon 2. By comparison with other reported members of the subfamily, the exon-intron boundaries also appear to be very well conserved. Further analysis allowed us to map this gene 1 Mb downstream from the HPRT gene in the published YAC contig that extends across Xq26. Two other motifs, 87 and 78% homologous to ZNF75 at the amino acid level, were identified by PCR on total human DNA, but map outside Xq24-qter. © 1993 by Academic Press, Inc.

引用

页码：223 / 229

页数：7

共 25 条

[1] YEAST ARTIFICIAL CHROMOSOMES CONTAINING HUMAN XQ24-XQ28 DNA - LIBRARY CONSTRUCTION AND REPRESENTATION OF PROBE SEQUENCES [J].