AGE-RELATED INCREASE IN METHYLATION OF RIBOSOMAL GENES AND INACTIVATION OF CHROMOSOME-SPECIFIC RIBOSOMAL-RNA GENE CLUSTERS IN MOUSE

被引：63

作者：

SWISSHELM, K ^{[1
]}

DISTECHE, CM ^{[1
]}

THORVALDSEN, J ^{[1
]}

NELSON, A ^{[1
]}

SALK, D ^{[1
]}

机构：

[1] UNIV WASHINGTON, DEPT PATHOL, SEATTLE, WA 98195 USA

来源：

MUTATION RESEARCH | 1990年 / 237卷 / 3-4期

基金：

美国国家卫生研究院;

关键词：

(Aging); (Mouse); Methylation; Nucleolar organizing regions; rRNA genes;

D O I：

10.1016/0921-8734(90)90019-N

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

An age-related increase in DNA methylation of the multicopy 18S and 28S ribosomal RNA genes was found in CBA/Ca mice beginning between 6 and 18 months of age at the 5′ end of these genes in liver, brain and spleen. The highest level of age-associated hypermethylation was mapped to the proximal 5′ spacer domain. Silver staining of actively transcribing ribosomal genes in metaphase chromosomes from stimulated spleen cells provided cytological evidence that these mice have 3 rRNA cistrons located on chromosomes 15, 16, and 18. The ribosomal gene cluster located on chromosome 16 was preferentially inactivated in older animals. Exposure of spleen cells from older individuals to 5-azacytidine appeared to both reactivate ribosomal gene clusters and reduce rRNA gene methylation. © 1990.

引用

页码：131 / 146

页数：16

共 68 条

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