DEVELOPMENT OF THE H-POINT STANDARD-ADDITIONS METHOD FOR ULTRAVIOLET VISIBLE SPECTROSCOPIC KINETIC-ANALYSIS OF 2-COMPONENT SYSTEMS

This work establishes the fundamentals of the H-point standard-additions method (HPSAM) to kinetic-data for the simultaneous determination of binary mixtures or the calculation of analyte concentrations, completely free from bias error. Two variants of the method are proposed; one is applied when the reaction of one component is faster than that of the other or the latter does not take place at all; the other is used when the rate constant of the two components are time-dependent. Also It is demonstrated that the shown accuracy of the method allows its application as a diagnostic tool for the reliability of analytical results. The method was applied to the determination of manganese and vanadium by simultaneous oxidation of Pyrogallol Red and also to the determination of creatinine in serum samples and to the simultaneous determination of creatinine and albumin by the Jaffe method.