A NOVEL ENZYME-IMMUNOASSAY FOR QUANTITATION OF RAT PROTHROMBIN IN MICROSOMAL SUBFRACTIONS

被引：5

作者：

MYRSET, AH

HELGELAND, L

机构：

[1] Department of Biochemistry, University of Oslo

来源：

JOURNAL OF IMMUNOASSAY | 1991年 / 12卷 / 04期

关键词：

ELISA; PROTHROMBIN PURIFICATION; ANTISERUM; IGG-PURIFICATION; AMIDOLYTIC ACTIVITY;

D O I：

10.1080/01971529108053282

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An enzyme linked immunoassay (ELISA) for quantitation of rat prothrombin, based on a biotin-streptavidin alkaline phosphatase system is described. The assay utilizes rabbit antiserum raised against purified rat prothrombin. The assay was twenty fold more sensitive than a rat prothrombin assay based on amidolytic activity following activation by Echis carinatus venom. Results obtained with the two assays show good correlation. The ELISA is a valuable tool for quantitation of minute amounts of prothrombin in subcellular fractions and large series of plasma samples.

引用

页码：597 / 609

页数：13

共 8 条

[1] RAT PROTHROMBIN - PURIFICATION, CHARACTERIZATION, AND ACTIVATION [J].