IMMUNOPRECIPITATION OF A PERTUSSIS TOXIN SUBSTRATE OF THE G(O) FAMILY FROM RAT ISLETS OF LANGERHANS

被引：3

作者：

BERROW, NS ^{[1
]}

HURST, RD ^{[1
]}

CHAN, SLF ^{[1
]}

MORGAN, NG ^{[1
]}

机构：

[1] UNIV TORONTO,DEPT MED,TORONTO M5S 1A8,ONTARIO,CANADA

来源：

BIOSCIENCE REPORTS | 1992年 / 12卷 / 02期

关键词：

INSULIN SECRETION; PANCREATIC B-CELL; G-PROTEIN; ADENYLATE CYCLASE;

D O I：

10.1007/BF02351213

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rat islets express a pertussis toxin sensitive G-protein involved in receptor-mediated inhibition of insulin secretion. This has been assumed previously to represent "G(i)" which couples inhibitory receptors to adenylate cyclase. Incubation of islet G-proteins with P-32-NAD and pertussis toxin resulted in the labelling of a band of molecular weight 40,000. This band was very broad and did not allow resolution of individual components. Incubation of the radiolabelled proteins with an anti-G(o) antiserum resulted in specific immunoprecipitation of a P-32-labelled band. These results demonstrate that the complement of pertussis toxin sensitive G-proteins in rat islets includes G(o).

引用

页码：95 / 100

页数：6

共 25 条

[1]

BERROW N S, 1990, Diabetologia, V33, pA87

[2]

BERROW NS, 1991, J MOL ENDOCRINOL, V8, P103

[3] MUSCARINIC-AGONIST AND GUANINE-NUCLEOTIDE ACTIVATION OF POLYPHOSPHOINOSITIDE PHOSPHODIESTERASE IN ISOLATED ISLET-CELL MEMBRANES [J].