TISSUE-SPECIFIC REGULATION OF BOVINE HEART CYTOCHROME-C-OXIDASE ACTIVITY BY ADP VIA INTERACTION WITH SUBUNIT-VIA

被引：105

作者：

ANTHONY, G

REIMANN, A

KADENBACH, B

机构：

[1] Fachbereich Chemie (Biochemie), Philipps-Universität, D-3550 Marburg, Hans-Meerwein-Strasse

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 05期

关键词：

CONFORMATIONAL CHANGE; ALLOSTERIC EFFECTOR; CROSS-REACTING MONOCLONAL ANTIBODY; NUCLEAR-CODED SUBUNIT;

D O I：

10.1073/pnas.90.5.1652

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The activity of reconstituted cytochrome-c oxidase (EC 1.9.3.1) from bovine heart is stimulated by intra-liposomal ADP but not by NaCl of the same ionic strength. A monoclonal antibody which reacts with subunits VIa-H (heart-type) and VIc, due to the evolutionary relationship between these subunits, also stimulates the activity of the enzyme from bovine heart but not from bovine liver. The antibody induces a conformational change in the heart enzyme but not in the liver enzyme, as shown by the visible difference spectrum. Preincubation of heart cytochrome-c oxidase with the antibody prevents stimulation of activity by intraliposomal ADP after reconstitution in liposomes. Reconstituted liver cytochrome c oxidase is not stimulated by intraliposomal ADP. The data suggest tissue-specific regulation of the activity of cytochrome-c oxidase by ADP via interaction with the matrix domain of subunit VIa-H.

引用

页码：1652 / 1656

页数：5

共 48 条

[1] ATP-INDUCED SPECTRAL CHANGES IN CYTOCHROME-C OXIDASE - A KINETIC INVESTIGATION [J].