A NOVEL METHOD FOR DETECTING ACETYLGLUCOSAMINIDASE ACTIVITY ON POLYACRYLAMIDE GELS

被引：3

作者：

LINCOLN, SP

FERMOR, TR

WOOD, DA

机构：

[1] Horticulture Research International, Department of Microbial Biotechnology, BN17 6lP, Worthing Road, Littlehampton, West Sussex

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1994年 / 20卷 / 02期

关键词：

POLYACRYLAMIDE GEL ELECTROPHORESIS; 4-METHYLUMBELLIFERYL N-ACETYL-BETA-D-GLUCOSAMINIDE; ACETYLGLUCOSAMINIDASE; ISOELECTRIC FOCUSING; AGARICUS BISPORUS;

D O I：

10.1016/0167-7012(94)90010-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Acetylglucosaminidase (EC 3.2.1.30) activity could be detected after SDS PAGE, non-SDS PAGE and isoelectric focusing. After SDS PAGE, enzyme activity was restored by washing in Triton X-100. Detection of acetylglucosaminidase bands was by incorporating in the gel, or incubation with, 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide.

引用

页码：79 / 85

页数：7

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