SYNTHESIS OF NITRIC-OXIDE RELEASING, VASODILATING AND PLATELET-AGGREGATION INHIBITING S-[N-15]NITROSO COMPOUNDS

[N-15]Nitric oxide ((NO)-N-15) was produced in a ''gas-tight'' flask from [N-15]nitrite by reaction with iodide in acetic acid acidified water and purged for 60 min by a continuous nitrogen gas stream applied through an uncoated polytetrafluoroethylene flate membrane into a second flask which contained a methanolic solution of N-acetyl-L-cysteine or N-acetyl-DL-penicillamine. Analysis of these solutions by UV spectroscopy, reversed-phase high-performance liquid chromatography and capillary isotachophoresis showed formation of the corresponding S-nitroso compounds. Gas chromatographic-mass spectrometric analysis for [N-15]nitrite which was formed by dissolving these compounds in aqueous buffered solutions gave an isotopic purity higher than 95% at N-15, The S-nitroso compounds were shown to inhibit ADP-induced platelet aggregation.