CELL-FREE SYNTHESIS OF THE MITOCHONDRIAL ADP-ATP CARRIER PROTEIN OF NEUROSPORA-CRASSA

被引：125

作者：

ZIMMERMAN, R ^{[1
]}

PALUCH, U ^{[1
]}

SPRINZL, M ^{[1
]}

NEUPERT, W ^{[1
]}

机构：

[1] MAX PLANCK INST EXPTL MED, CHEM ABT, D-3400 GOTTINGEN, GERMANY

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1979年 / 99卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1979.tb13251.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ADP/ATP carrier protein was synthesized in heterologous cell‐free systems programmed with Neurospora poly(A)‐containing RNA and homologous cell‐free systems from Neurospora. The apparent molecular weight of the product obtained in vitro was the same as that of the authentic mitochondrial protein. The primary translation product obtained in reticulocyte lysates starts with formylmethionine when formylated initiator methionyl‐tRNA (fMet‐tRNAfMet) was present. The product synthesized in vitro was released from the ribosomes into the postribosomal supernatant. The evidence presented indicates that the ADP/ATP carrier is synthesized as a polypeptide with the same molecular weight as the mature monomeric protein and does not carry an additional sequence. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：247 / 252

页数：6

共 33 条

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