PRECISE QUANTITATION OF GENE NUMBER BY SATURATION HYBRIDIZATION USING CLONED DNA

被引：5

作者：

LONGACRE, SS

MACH, B

机构：

[1] Department of Microbiology, University of Geneva Medical School, 1205 Geneva, 64, Avenue de la Roseraie

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 6卷 / 04期

关键词：

D O I：

10.1093/nar/6.4.1241

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper describes a precise method of gene titration as applied to the α- and β-globin genes in the mouse. The three salient features of the method are: (i) the use of saturation hybridization in probe cDNA excess, (ii) the use of highly purified cDNA probes prepared by preparative hybridization with cloned globin sequences (Longacre and Mach (1978) J. Biol. Chem. 253, 7500) and (iii) the use of cloned globin sequences to calibrate the system internally. The results indicate that there are two genes for α-globin and two genes for β-globin in the BALB/c mouse. The significance of these results are discussed in relation to other data regarding adult and embryonic globin genes. © 1979 Information Retrieval Limited.

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页码：1241 / 1258

页数：18

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